By John N. Abelson, Melvin I. Simon, James A. McCloskey
This quantity offers a compilation of experimental equipment and ideas, with precise protocols and examples. options that have confirmed worth in problem-solving are integrated. meant for biochemists, cellphone biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists, analytical chemists, and physiologists, this ebook makes a speciality of mass spectrometry. subject matters mentioned comprise the equipment of ionization, liquid chromatography, non-stop movement quickly atom bombardment mass spectrometry, collision-induced dissociation, enzymatic and chemical digestion of proteins, and components of nucleic acids. different titles during this sequence comprise Guide to Yeast Genetics and Molecular Biology and Retinoids, half B: telephone Differentiation and scientific purposes .
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Extra info for Mass Spectrometry
36 GENERAL TECHNIQUES  lyzer. With this technique, molecular masses in excess of 100 kDa have been recorded. Future Directions With current, rapid explosion of new ionization techniques it is difficult to predict the future and the impact that mass spectrometry will have on structural analysis of large, more complex, biomolecules. The fact that high mass molecular ions are observed by such widely disparate techniques as UV laser desorption (for which singly charged ions predominate) and electrospray (which produces multiply charged ions) attests to the unusual stability of biological macromolecules in the gas phase as well as in solution.
McLaren, Rev. Sci. Instrum. 26, 1150 (1955). 32 A. Benninghoven, E. Niehuis, T. Friese, D. Griefendorf, and P. Steffens, Org. Mass Spectrom. 19, 346 (1984). 33 R. D. Mcfarlane, Anal. Chem. 55, 1247A (1983). 5#s VOLTAGE FIG. 12. Schematic of the Wiley-McLaren TOF mass spectrometer) tion followed by multiphoton ionization, 34 the ionization plane is well defined, and much higher resolving power can be obtained. The thermal energy of sample molecules formed by laser desorption has also been reduced by using a supersonic jet before generating ions by multiphoton ionization.
Bonner, B. I. Shushan, and J. Henion, Rapid Commun. Mass Spectrom. 2, 249 (1988). 95 R. D. Smith, C. J. Berinaga, and H. R. Udseth, Anal. Chem. 60, 1948 (1988). 36 GENERAL TECHNIQUES  lyzer. With this technique, molecular masses in excess of 100 kDa have been recorded. Future Directions With current, rapid explosion of new ionization techniques it is difficult to predict the future and the impact that mass spectrometry will have on structural analysis of large, more complex, biomolecules. The fact that high mass molecular ions are observed by such widely disparate techniques as UV laser desorption (for which singly charged ions predominate) and electrospray (which produces multiply charged ions) attests to the unusual stability of biological macromolecules in the gas phase as well as in solution.